Development and characterization of an alternative control system to study separately oxygen and momentum transfer effects in stirred tank bioreactors

Control of dissolved oxygen tension (DOT) in stirred tank bioreactors (STB) is commonly performed by manipulation gas flow or agitation. However, control systems using this strategy directly alter hydrodynamic conditions thus interrelating and momentum transfer effects the culture. The aim study was to develop evaluate an alternative DOT system algorithm that allows further separately. A novel designed with three mass controllers manipulating partial pressure  The developed tightly controlled at 30% a 3-L bioreactor Escherichia coli, maintaining constant agitation.  energy dissipation rate (EDR) agitation maintained 0.06 W/kg, EDR variations via bubble wake bursting were diminished. accumulated error 42% lower than standard commercial controller. Additionally, effectively responded perturbations on set point (30, 50, 80%) (200, 500, 1000 rpm). To best authors’ knowledge, first characterization controller terms could be used separately STB.&nbsp